Browsing by Author "Campo Sfeir, Andrea del"

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    Multi-scale mitochondrial cristae remodeling links Opa1 downregulation to reduced OXPHOS capacity in aged hearts
    (2025) Molina Riquelme, Isidora Elvira; Barrientos Guajardo, Gonzalo Andres; Breitsprecher, Leonhard; Gomez Calderon, Wileidy Andrea; Diaz Castro, Francisco Leopoldo; Morris, Silke; Campo Sfeir, Andrea del; Garrido Olivares, Luis Eugenio; Verdejo Pinochet, Hugo; Psathaki, Olympia Ekaterini; Busch, Karin B.; Eisner Sagüés, Verónica Raquel
    Aging is closely associated with cardiovascular diseases, the leading cause of mortality worldwide.Mitochondrial dysfunction is a hallmark of cardiovascular aging because it generates most of the heart's ATP at the cristae, specialized sub-compartments where OXPHOS takes place. In this study, we used multiple-scale electron microscopy approaches to evaluate age-related mitochondrial and cristaeultrastructural alterations in human and mouse hearts. We found that aged patients’ hearts displayed reduced cristae density as seen by TEM, even before any significant decline in the expression of cristae-shaping proteins. Similarly, a multi-scale approach that included SBF-SEM and TEM showed that in aged mice’s hearts cristae undergo ultrastructural remodeling processes, resulting in a decrease in cristae density and width. Electron tomography suggests an apparent decline in cristae connectivity, and an increase in fenestration size. These changes were linked to Opa1 downregulation, accompanied by reduced OXPHOS maximal respiration, but unrelated to alterations in the levels of OXPHOS core subunits and ATP synthase assembly. Altogether, this indicates that alterations in cristae structure alone are sufficient to impair oxidative metabolism, which highlights its potential as an early signal of cardiac aging, even before noticeable changes in mitochondrial morphology occur.