Browsing by Author "Campino, C"

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    Bilateral oophorectomy in a pregnant woman
    (2005) Villaseca, P; Campino, C; Oestreicher, E; Mayerson, D; Serón-Ferré, M; Arteaga, E
    Background: A 16 week pregnant woman presented with massive theca-lutein cysts requiring bilateral oophorectomy. Pregnancy progressed uneventfully and spontaneous lactation ensued after delivery. Methods: To study the role of the ovary on the hormonal profile at the end of gestation and in post-partum, we measured FSH, estradiol (E-2), unconjugated estrone (E-1), unconjugated estriol (E-3), sex hormone-binding globulin, progesterone, dehydroepiandrosterone sulphate and prolactin at 37 weeks gestation and at 8 h, 4 days, 5 weeks, and 2 months post-partum. Results: These hormones were within the range expected for ovary-intact pregnant and puerperal women until 4 days post-partum. At 5 weeks post-partum, FSH increased to a peri-menopausal range (31.4 IU/l) while estrogens remained within the normal puerperal range (E-2=239 pmol/l; E-1=102 pmol/l), contrasting with their rapid changes in non-pregnant women after bilateral oophorectomy. At 2 months, while partially breastfeeding, FSH, E-2 and E-1 were closer to menopausal range (68 IU/l, 136 and 70.2 pmol/l respectively), and hormone replacement was started. Conclusions: We conclude that the ovary is not required to maintain a normal hormonal profile in late pregnancy and early puerperium. However, the increase in FSH to peri-menopausal levels at 5 weeks post-partum, despite breastfeeding, suggests that the ovary is needed to maintain low FSH concentrations during lactation.
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    Bioactivity of prolactin isoforms
    (1999) Campino, C; Torres, C; Ampuero, S; Díaz, S; González, GB; Serón-Ferré, M
    To assess whether plasma prolactin (PRL) characteristics relate to lactogenesis and absence or presence of menstrual cycles, we measured bioactive PRL (BIO;PRL) using the Nb2 assay, immunoreactive PRL (TR-PRL) by radioimmunoassay, calculated equations describing the BIO-PRL-IR-PRL relationship and separated charged PIiL isoforms (by chromatofocusing) in five amenorrhoeic and five cycling nursing women at 6 months postpartum and in 10 cycling non-nursing women, Plasma samples were drawn before and 30 min after a suckling episode at 0800, 1600 and 2400 h in nursing women and at the same hours ih non-nursing women. BIO-PRL and IR-PRL concentrations were highest in amenorrhoeic nursing women, intermediate in cycling nursing women and lowest in cycling non-nursing women. The BIO-PRL-IR-PRL relationship shows that a given amount of IR-PRL corresponds to equivalent amounts of BIO-PRL in cycling nursing and cycling nonnursing women, and to a larger extent in amenorrhoeic nursing women. IR-PRL was present In plasma as several charge isoforms, Bioactive isoforms eluting at PH 6.0-5.1 were found in amenorrhoeic and cycling nursing women, reaching similar concentrations after suckling. Bioactive isoforms eluting at pH 7.0-6.1 were found only in amenorrhoeic nursing women. We speculate that isoforms eluting at pH 6.0-5.1 may play a role in lactation and isoforms eluting at pH 7.0-6.1, in lactational amenorrhoea.
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    Biochemical and genetic characterization of 11 beta- hydroxysteroid dehydrogenase type 2 in low-renin essential hypertensives
    (LIPPINCOTT WILLIAMS & WILKINS, 2005) Carvajal, CA; Romero, DG; Mosso, LM; Gonzalez, AA; Campino, C; Montero, J; Fardella, CE
    Background The 11beta-hydroxysteroid dehydrogenase type 2 (11betaHSD2) catalyzes the conversion of cortisol M to cortisone (E), avoiding the interaction of cortisol with the mineralocorticoid receptor. If it fails, cortisol will stimulate sodium and water reabsorption, increasing the intravascular volume that suppresses renin and secondarily increase the blood pressure.
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    Bone turnover and density in healthy women during breastfeeding and after meaning
    (1996) Lopez, JM; Gonzalez, G; Reyes, V; Campino, C; Diaz, S
    To investigate the changes in maternal bone density and turnover associated with lactation we ran a longitudinal study in fully breastfeeding women (age 26.3+/-4.1 years, mean+/-SD) at the first (stage I, n = 30) and sixth (stage II, n = 25) months postpartum and 6 months after weaning (stage III, n = 20), and in a contemporary control group of non-nursing women. At each time point bone density, serum calcium, phosphorus, alkaline phosphatases, parathyroid hormone (PTH), osteocalcin, follicle stimulating hormone (FSH), estradiol (E(2)), prolactin (PRL) urinary hydroxyproline and creatinine (OH-P/Cr) were measured in both groups. The daily calcium intake of nursing women (1479 +/- 590 mg/day at stage I) was higher than in non-nursing women (536 +/- 231 mg/day at stage I). Biochemical markers of bone turnover were higher (p<0.05) in nursing than in non-nursing women at stages I and II, while in stage III only OH-P/Cr was elevated. The lumbar spine (L2-4) bone mineral density was similar in the two groups at the beginning of the study (1.148 +/- 0.111 g/cm(2) in nursing women vs 1.211 +/- 0.102 g/cm(2) in non-nursing women; p = 0.56), but it was lower in nursing women at stage II (1.144 +/- 0.110 g/cm(2) vs 1.216 +/- 0.095 g/cm(2) respectively; p<0.05). Right cm femoral neck bone density decreased by 3% between stages I and II in nursing women but did not differ from values in non-nursing women (0.947 +/- 0.110 vs 0.973 +/- 0.108 in stage I and 0.918 +/- 0.114 vs 0.975 +/- 0.098 in stage II respectively; p<0.05, ANOVA). After weaning, lumbar spine and femoral neck bone density increased by 6% and 8% respectively (p<0.05, ANOVA). No correlation was found between changes in bone turnover markers or bone density and parity, frequency and duration of nursing episodes, body weight, body mass index, and plasma PRL, E(2) and PTH levels. We conclude that in nursing women with a daily calcium intake at the recommended dietary allowance (>1200 mg/day), full breastfeeding extending over 6 months is characterized by increased maternal bone turnover and a transient bone loss which normalizes after weaning.
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    Norplant® implants and progesterone vaginal rings do not affect maternal bone turnover and density during lactation and after weaning
    (1999) Díaz, S; Reyes, MV; Zepeda, A; González, GB; López, JM; Campino, C; Croxatto, HB
    Bone density and turnover was assessed in a longitudinal study of healthy lactating women who initiated use of Norplant(R) implants (NOR, n = 29), progesterone vaginal rings (PVR, n = 28) or Copper T 380A intrauterine devices (T-Cu, n = 51, control group) around day 60 postpartum. Bone density, serum calcium, phosphorus, alkaline phosphatases, parathyroid hormone (PTH), follicle stimulating hormone (FSH), oestradiol and prolactin, and urinary hydroxyproline and creatinine were measured at postpartum months 1 (PM1), and 12 (PM12) and 6 or 12 months after weaning; at month 6 postpartum (PM6) serum and urine tests alone were performed. Baseline characteristics and lactation performance were similar between groups. Biochemical markers of bone turnover were higher at PM1, PM6 and PM12 than after weaning, with no differences between groups. Bone density in the lumbar spine (L2-L4) and femoral neck at PM1 and PM12 (similar to 1.11 g/cm(2)) was similar in three groups. Lumbar spine values were found to be lower in lactating women than those present in nonlactating women, but increased after weaning to similar values, The two progestin-only contraceptives studied appear to have no deleterious effect upon bone density and metabolism in healthy lactating women.
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    Post-suckling prolactin:oestradiol ratio - a potential index to predict the duration of lactational amenorrhoea in women
    (1997) Campino, C; Ampuero, S; Diaz, S; Lopez, JM; SeronFerre, M
    To assess whether the duration of lactational amenorrhoea can be predicted in individual women, we studied the pre-and post-suckling concentrations of immune prolactin (IR-PRL) and of bioactive prolactin (BIO-PRL) and basal concentrations of oestradiol in ten amenorrhoeic fully nursing women at 3 months post-partum. The women were of similar age, weight and had infants of similar growth rate, Five of these women were to experience long amenorrhoea (>180 days) and the others short amenorrhoea (<180 days), Blood samples were drawn 30 min after a suckling episode initiated at 0800 h, 1600 h and 2400 h. BIO-PRL distinguished between groups of women at 0030 h but not at other times, while there was considerable overlap between values for IR-PRL and oestradiol at all times studied, At 1630 h, the ratios post-suckling BIO-PRL: oestradiol and post-suckling IR-PRL:oestradiol were above 2000 in the women that were to experience long amenorrhoea and below this threshold in the other women, The ratio post-suckling BIO-PRL:oestradiol provided more information since the difference between the lowest ratio in the long amenorrhoea and the highest ratio in the short was 699, while it was 520 for the IR-PRL:oestradiol ratio, The determination of these ratios may help to predict the duration of lactational amenorrhoea in individual fully nursing women.