Browsing by Author "Camilo, Conrado"
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- ItemA macroporous resin purification process to obtain food-grade phlorotannin-rich extracts with a-glucosidase inhibitory activity from Chilean brown seaweeds: An UHPLC-MSn profiling(2023) Erpel, Fernanda; Camilo, Conrado; Mateos, Raquel; Perez Correa, Jose Ricardo
- ItemChemical evidence of prehistoric passive tobacco consumption by a human perinate (early Formative Period, South-Central Andes)(2018) Niemeyer, Hermann M.; de Souza, Patricio; Camilo, Conrado; Echeverria, Javier
- ItemHeterologous production of the epoxycarotenoid violaxanthin in Saccharomyces cerevisiae(2020) Cataldo, Vicente F.; Arenas, Natalia; Salgado, Valeria; Camilo, Conrado; Ibanez, Francisco; Agosin, EduardoMicrobial production of carotenoids has mainly focused towards a few products, such as beta-carotene, lycopene and astaxanthin. However, other less explored carotenoids, like violaxanthin, have also shown unique properties and promissory applications. Violaxanthin is a plant-derived epoxidated carotenoid with strong antioxidant activity and a key precursor of valuable compounds, such as fucoxanthin and beta-damascenone. In this study, we report for the first time the heterologous production of epoxycarotenoids in yeast. We engineered the yeast Saccharomyces cerevisiae following multi-level strategies for the efficient accumulation of violaxanthin. Starting from a beta-carotenogenic yeast strain, we first evaluated the performance of several beta-carotene hydroxylases (CrtZ), and zeaxanthin epoxidases (ZEP) from different species, together with their respective N-terminal truncated variants. The combined expression of CrtZ from Pantoea ananatis and truncated ZEP of Haematococcus lacustris showed the best performance and led to a yield of 1.6 mg/g(DCW) of violaxanthin. Further improvement of the epoxidase activity was achieved by promoting the transfer of reducing equivalents to ZEP by expressing several redox partner systems. The co-expression of the plant truncated ferredoxin-3, and truncated root ferredoxin oxidoreductase-1 resulted in a 2.2-fold increase in violaxanthin yield (3.2 mg/g(DCW)). Finally, increasing gene copy number of carotenogenic genes enabled reaching a final production of 7.3 mg/g(DCW) in shake flask cultures and batch bioreactors, which is the highest yield of microbially produced violaxanthin reported to date.
- ItemMulti-response optimal hot pressurized liquid recovery of extractable polyphenols from leaves of maqui (Aristotelia chilensis [Mol.] Stuntz)(2021) Rivera-Tovar, Pamela Raquel; Torres, Maria Dolores; Camilo, Conrado; Mariotti-Celis, Maria Salome; Dominguez, Herminia; Perez Correa, Jose Ricardo