Browsing by Author "CROSS, D"
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- ItemBIOSYNTHESIS OF THE NEUROFILAMENT HEAVY SUBUNIT IN XENOPUS OOCYTES MICROINJECTED WITH RAT-BRAIN POLY(A)+ RNA(1987) CROSS, D; ALLENDE, ML; KRAUSS, RY; FUENTES, ME; KALTWASSER, G; ALVAREZ, J; INESTROSA, NCIn order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.