Browsing by Author "Boyer, Joshua D."

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    Implementation of Mass Cytometry as a Tool for Mechanism of Action Studies in Inflammatory Bowel Disease
    (2018) Tyler, Christopher J.; Perez-Jeldres, Tamara; Ehinger, Erik; Capaldo, Brian; Karuppuchamy, Thangaraj; Boyer, Joshua D.; Patel, Derek; Dulai, Parambir; Boland, Brigid S.; Lannigan, Joanne; Eckmann, Lars; Ernst, Peter B.; Sandborn, William J.; Ho, Samuel B.; Rivera-Nieves, Jesus
    Background: Novel therapeutics for inflammatory bowel disease (IBD) are under development, yet mechanistic readouts at the tissue level are lacking. Techniques to assess intestinal immune composition could represent a valuable tool for mechanism of action (MOA) studies of novel drugs. Mass cytometry enables analysis of intestinal inflammatory cell infiltrate and corresponding molecular fingerprints with unprecedented resolution. Here, we aimed to optimize the methodology for isolation and cryopreservation of cells from intestinal tissue to allow for the potential implementation of mass cytometry in MOA studies.