Browsing by Author "Bernal, Giuliano"

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    Annona cherimola Seed Extracts Trigger an Early Apoptosis Response and Selective Anticlonogenic Activity against the Human Gastric Carcinoma Cell Line SNU-1
    (2023) Macuer-Guzman, Johan; Giovagnoli-Vicuna, Claudia; Bernal, Giuliano; Lobos-Gonzalez, Lorena; de la Fuente-ortega, Erwin; Araya-Castillo, Michael; Ibanez, Cristian
    The aim of this study was to evaluate, for the first time, the antiproliferative, apoptotic and diminishing effects of the anchored growth-independent capacity of an ethanol macerate extract from the Annona cherimola seed (EMCHS) in the human gastric cancer cell line SNU-1. The cells treated with EMCHS (20 mu g/mL) significantly reduced the capacity to form clones of the tumor cell. Moreover, 50 mu g/mL of EMCHS extract induced apoptosis, as was shown by the Annexin-V assay. UHPLC-MS/MS analysis detected two acetogenins (Annonacinone and Annonacin) in the EMCHS, which could be largely responsible for its selective antiproliferative effect. The identification of fatty acids by GC-FID showed the presence of eight fatty acids, among which was, oleic acid, which has recognized activity as an adjuvant in antitumor treatments. Taken together, our results indicate that the EMCHS seems promising for use as a natural therapy against gastric cancer disease.
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    In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
    (NATURE RESEARCH, 2020) Guzman, Leda; Villalon, Katherine; Jose Marchant, Maria; Elena Tarnok, Maria; Cardenas, Pilar; Aquea, Gisela; Acevedo, Waldo; Padilla, Leandro; Bernal, Giuliano; Molinari, Aurora; Corvalan, Alejandro
    The cytotoxic mechanism of the saponin QS-21 and its aglycone quillaic acid (QA) was studied on human gastric cancer cells (SNU1 and KATO III). Both compounds showed in vitro cytotoxic activity with IC50 values: 7.1 mu M (QS-21) and 13.6 mu M (QA) on SNU1 cells; 7.4 mu M (QS-21) and 67 mu M (QA) on KATO III cells. QS-21 and QA induce apoptosis on SNU1 and KATO III, as demonstrated by TUNEL, Annexin-V and Caspase Assays. Additionally, we performed in silico docking studies simulating the binding of both triterpenic compounds to key proteins involved in apoptotic pathways. The binding energies (G(bin)) thus calculated, suggest that the pro-apoptotic protein Bid might be a plausible target involved in the apoptotic effect of both triterpenic compounds. Although QA shows some antiproliferative effects on SNU1 cells cultured in vitro, our results suggest that QS-21 is a more powerful antitumor agent, which merits further investigation regarding their properties as potential therapeutic agents for gastric cancer.