Browsing by Author "Benadof, D."

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    A new multiplex PCR assay for the simultaneous detection of vancomycin-resistant enterococci from rectal swabs
    (2010) Benadof, D.; San Martín, M.; Aguirre, J.; Paredes, L.; Malig, R.; Melo Ledermann, Francisco Javier; Lehouque, G.
    Objectives This study describes the diagnostic performance of a recently available multiplex PCR-based kit for the simultaneous detection and identification of Enterococcus faecium, Enterococcus faecalis, vanA, vanB, vanC1 and vanC2/C3 genes, directly from rectal swabs constituting the most complete existing molecular assay currently available. Methods The diagnostic performance of this assay was evaluated by a multicenter study involving three independent public hospitals and consisted in the analysis of 187 rectal swabs from patients at high risk for vancomycin-resistant enterococci colonization. Results When bacteria culture was used as the gold standard, the sensitivity, specificity, positive and negative predicted values for the assay were 96.8%, 76.0%, 67.7% and 97.9%, respectively. When a composite reference standard consisting of culture and DNA sequencing of PCR products was used as the gold standard, the sensitivity, specificity, positive and negative predicted values for the PCR-based assay were 97.8%, 96.9%, 96.7% and 97.9%, respectively. Conclusions Based on these results, we conclude that this assay is considerably more sensitive than traditional microbiological methods for detecting vancomycin-resistant enterococci from rectal swabs. It is also much faster than culture. We believe that the implementation of this assay in routine clinical laboratories could help to reduce hospital-acquired vancomycin-resistant enterococci infections.
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    Use of the WHO Access, Watch, and Reserve classification to define patterns of hospital antibiotic use (AWaRe): an analysis of paediatric survey data from 56 countries
    (2019) Hsia, Yingfen; Lee, Brian R.; Versporten, Ann; Yang, Yonghong; Bielicki, Julia; Jackson, Charlotte; Newland, Jason; Goossens, Herman; Magrini, Nicola; Sharland, Mike; Teston, L.; Cheung, K.; Koning, S.; Grimwood, K.; Cross, J.; da Silva, A.; Benadof, D.; Zhang, W. S.; Zhao, W.; Liu, G.; Shen, K. L.; Yao, K. H.; Zheng, Y. J.; Deng, J. K.; Zhang, J. S.; Wang, Y.; Jiang, X. Y.; Tian, D. Y.; Jing, C. M.; Wang, L. J.; Cao, S. C.; Wu, L. J.; Chen, X.; Ding, M. J.; Zhang, L.; Lin, L.; Yang, J. H.; Cao, Q.; Wang, W.; Li, J. P.; Tang, L. F.; Liu, J.; Wang, P.; Qian, J.; Zhu, C. M.; Lu, G.; Deng, Q. L.; Mu, X. P.; Zhao, C. A.; Dong, X. Y.; Zhang, H.; Li, C. Y.; Li, W.; Saxen, H.; Kekomaki, S.; Hufnagel, M.; Rippberger, B.; Knuf, M.; Nikolic, P.; Huebner, J.; Kreitmeyer, K.; Behrends, U.; Rieber, N.; Renk, H.; Spyridis, N.; Tsolia, M.; Papaevangelous, V; Gkentzi, D.; Syrogiannopoulos, G.; Kaffe, K.; Roilides, E.; Pitsava, G.; Papadimitriou, E.; Iosifidis, E.; Gandra, S.; Laxminarayan, R.; Alvarez-Uria, G.; Jinka, D.; Murki, Srinivas; Kandraju, H.; Singh, S.; Vasudevan, A. K.; Kanithi, R.; Akula, A.; Chikkappa, A.; Tunga, O.; Subramanian, S.; Sharma, A.; Dharmapalan, D.; Ashkenazi-Hoffnung, L.; Ashkenazi, S.; Esposito, S.; Tagliabue, C.; Tersigni, C.; Galli, L.; D'Argenio, P.; Pansa, P.; Duse, M.; Horikoshi, Y.; Fukuoka, K.; Jimenez, R.; Ojeda, K.; Okokon, I; Mahmood, H.; Gowin, E.; Slowinska-Jarzabek, B.; Majda-Stanislawska, E.; Sicinska, J.; Chan, S. M.; Chang, A.; Rozic, M.; Premru, M.; Finlayson, H.; Whitelaw, A.; Rabie, H.; Dramowski, A.; O'Connell, N.; Epalza, C.; Rojo-Conejo, Pablo; Martinon Torres, Federico; Justicia, Antonio; Jian, V; Cheng, C. L.; Lumbiganon, P.; Paopongsawan, P.; Puthanakit, T.; Anugulruengkitt, S.; Yarci, E.; Doerholt, K.; Vazouras, K.; Bamford, A.; Irwin, A.; Drysdale, S. B.; Collett-White, F.; Harkensee, C.; McMaster, P.; Green, H.; Rees, S.; Ledoare, K.; Chappell, F.; Jacqueline, D.; Hackett, S.; Vergnano, S.; Praveen, S.; Herberg, J.; Speirs, L.; Moriarty, Paul; Lacej, D.; Hoxha, I; Cornistein, W.; Quiros, R.; Hojman, M.; Del Castillo, M.; Ghazaryan, L.; AlSalman, J.; Konopnicki, D.; Pierard, D.; Holemans, X.; Schelstraete, P.; Firre, E.; Van Herendael, B.; Dedeic-Ljubovic, A.; Pignatari, A.; Sabuda, D.; Carvajal, C.; Alvaro, R.; Labarca, J.; Solano, A.; Ramirez, C.; Marekovic, I; Horvatic, J.; Pristas, I; Marshall, E.; Pagava, K.; Korinteli, I; Neubert, A.; Enimil, A.; Frimpong, J. A.; Soltani, J.; Fitzgerald, D.; Kasahara, K.; Gu, Y.; Okinaka, K.; Kunishima, H.; Elhajji, F. Darwish; Alshehri, M.; Raka, L.; Kambaralieva, B.; Sviestina, I; Burokiene, S.; Usonis, V; Shaqiri, E.; Zarb, P.; Markovic, G.; Simovic, S.; Nwajiobi-Princewil, P.; Iregbu, K.; Aboderin, A.; Oduyebo, O.; Olayinka, A.; McCorry, A.; McCullagh, B.; Gormley, C.; Rachina, S.; Carevic, B.; Chen, H. H.; Ling, M. L.; Terol Barrero, P.; Buijtels, P.; van Elzakker, E.; Thompson, S.; Cooper, M.; Rios, E.; Hudson, M.; Greer, N.; Gessner-Wharton, M.; Gawrys, G.
    Background Improving the quality of hospital antibiotic use is a major goal of WHO's global action plan to combat antimicrobial resistance. The WHO Essential Medicines List Access, Watch, and Reserve (AWaRe) classification could facilitate simple stewardship interventions that are widely applicable globally. We aimed to present data on patterns of paediatric AWaRe antibiotic use that could be used for local and national stewardship interventions.