Browsing by Author "Becker C., María Inés"
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- ItemA LIGHT-INDUCED TRYPTOPHAN-RIBOFLAVIN BINDING - BIOLOGICAL IMPLICATIONS(1991) Silva Stevens, Eduardo Andrés; Salim-Hanna, Marta; EdwardsM., Ana M; Becker C., María Inés; De Ioannes I., Alfredo E.
- ItemAllergens of the urushiol family promote mitochondrial dysfunction by inhibiting the electron transport at the level of cytochromes b and chemically modify cytochrome c1(2021) Pacheco, Rodrigo; Quezada, Sergio A.; Kalergis Parra, Alexis Mikes; Becker C., María Inés; Ferreira, Jorge; Ioannes, Alfredo E. deBackground: Urushiols are pro-electrophilic haptens that cause severe contact dermatitis mediated by CD8+ effector T-cells and downregulated by CD4+ T-cells. However, the molecular mechanism by which urushiols stimulate innate immunity in the initial stages of this allergic reaction is poorly understood. Here we explore the sub-cellular mechanisms by which urushiols initiate the allergic response. Results: Electron microscopy observations of mouse ears exposed to litreol (3-n-pentadecyl-10-enyl-catechol]) showed keratinocytes containing swollen mitochondria with round electron-dense inclusion bodies in the matrix. Biochemical analyses of sub-mitochondrial fractions revealed an inhibitory effect of urushiols on electron flow through the mitochondrial respiratory chain, which requires both the aliphatic and catecholic moieties of these allergens. Moreover, urushiols extracted from poison ivy/oak (mixtures of 3-n-pentadecyl-8,11,13 enyl/3-n-heptadecyl-8,11 enyl catechol) exerted a higher inhibitory effect on mitochondrial respiration than did pentadecyl catechol or litreol, indicating that the higher number of unsaturations in the aliphatic chain, stronger the allergenicity of urushiols. Furthermore, the analysis of radioactive proteins isolated from mitochondria incubated with 3H-litreol, indicated that this urushiol was bound to cytochrome c1. According to the proximity of cytochromes c1 and b, functional evidence indicated the site of electron flow inhibition was within complex III, in between cytochromes bL (cyt b566) and bH (cyt b562). Conclusion: Our data provide functional and molecular evidence indicating that the interruption of the mitochondrial electron transport chain constitutes an important mechanism by which urushiols initiates the allergic response. Thus, mitochondria may constitute a source of cellular targets for generating neoantigens involved in the T-cell mediated allergy induced by urushiols.
- ItemAn alternative ELISA for T4 determination based on idiotype anti-idiotype interaction and a latex method for anti-Idiotype monoclonal antibody selection(1996) Becker C., María Inés; Foradori, Arnaldo; De Ioannes I., Alfredo E.
- ItemApoptosis induction in nonirradiated human HL-60 and murine NSO/2 tumor cells by photoproducts of indole-3-acetic acid and riboflavin(1999) EdwardsM., Ana M; Barredo, F.; Silva, E.; De Ioannes I., Alfredo E.; Becker C., María Inés
- ItemChemosensitizing Effect Of Nordihydroguaiaretic Acid (Ndga) And Its Tetra Acetylated Derivative (Ndgata) On Parental And Multiresistant Ta3 Mouse Mammary Adenocarcinoma Cell Lines(2008) Ferreira, Jorge; Pavani, Mario; Jana, Fabian; Burgos, Paula I.; Morello, Antonio; Maya, Juan Diego; Faúndez Cáceres, Mario; Lopez, Americo; De Ioannes I., Alfredo E.; Becker C., María Inés
- ItemDifferent cell death mechanisms are induced by a hydrophobic flavin in human tumor cells after visible light irradiation(2011) Muñoz Figueroa, Marcelo Alejandro; Pacheco Díaz, Ariadna; Becker C., María Inés; Silva, Eduardo; Ebensperger González, Roberto Alejandro; García Torres, Angélica María; De Ioannes I., Alfredo E.; EdwardsM., Ana M
- ItemFemales of the communally breeding rodent, Octodon degus, transfer antibodies to their offspring during pregnancy and lactation(2007) Becker C., María Inés; De Ioannes I., Alfredo E.; Ebensperger Pesce, Luis Alberto
- ItemImmunodetection of acrosin during the acrosome reaction of hamster, guinea-pig and human spermatozoa(1992) Barros Rodríguez, Fernando Claudio; Becker C., María Inés; De Ioannes I., Alfredo E.
- ItemInhibitory effect of nordihydroguaiaretic acid and its tetra-acetylated derivative on respiration and growth of adenocarcinoma TA3 and its multiresistant variant TA3MTX-R(2008) Plaza, Claudio; Pavani, Mario; Faúndez Cáceres, Mario; Maya, Juan Diego; Morello, Antonio; Becker C., María Inés; De Ioannes I., Alfredo E.; Cumsille, Miguel Angel; Ferreira, Jorge
- ItemMucin Allows Survival of Salmonella Typhi Within Mouse Peritoneal Macrophages(1993) Sein Pavéz, Jorge Daniel.; Cachicas Cubillos, Viviana Eugenia.; Becker C., María Inés; De Ioannes I., Alfredo E.
- ItemOxidative modifications in crystallin proteins and lens epithelial cells associated with photosensitized reactions mediated by the major chromophore arising from glucose degradation(2016) Vargas Brancoli, Francisco José; Becker C., María Inés; Friguet, Bertrand; Silva S., E.
- ItemReactivity of monoclonal antibodies against a tryptophan-riboflavin adduct toward irradiated and non-irradiated bovine-eye-lens protein fractions: an indicator of in vivo visible-light-mediated phototransformations(2000) Mancini, Marcelo; EdwardsM., Ana M; Becker C., María Inés; De Ioannes I., Alfredo E.; Silva Stevens, Eduardo Andrés
- ItemSegregation of Cell Surface Antigens During Early Development of mouse Gives Positional Information for Blastocyst Differentiation(1993) Becker C., María Inés; De Ioannes I., Alfredo E.