• La Universidad
    • Historia
    • Rectoría
    • Autoridades
    • Secretaría General
    • Pastoral UC
    • Organización
    • Hechos y cifras
    • Noticias UC
  • 2011-03-15-13-28-09
  • Facultades
    • Agronomía e Ingeniería Forestal
    • Arquitectura, Diseño y Estudios Urbanos
    • Artes
    • Ciencias Biológicas
    • Ciencias Económicas y Administrativas
    • Ciencias Sociales
    • College
    • Comunicaciones
    • Derecho
    • Educación
    • Filosofía
    • Física
    • Historia, Geografía y Ciencia Política
    • Ingeniería
    • Letras
    • Matemáticas
    • Medicina
    • Química
    • Teología
    • Sede regional Villarrica
  • 2011-03-15-13-28-09
  • Organizaciones vinculadas
  • 2011-03-15-13-28-09
  • Bibliotecas
  • 2011-03-15-13-28-09
  • Mi Portal UC
  • 2011-03-15-13-28-09
  • Correo UC
- Repository logo
  • English
  • Català
  • Čeština
  • Deutsch
  • Español
  • Français
  • Gàidhlig
  • Latviešu
  • Magyar
  • Nederlands
  • Polski
  • Português
  • Português do Brasil
  • Suomi
  • Svenska
  • Türkçe
  • Қазақ
  • বাংলা
  • हिंदी
  • Ελληνικά
  • Yкраї́нська
  • Log in
    Log in
    Have you forgotten your password?
Repository logo
  • Communities & Collections
  • All of DSpace
  • English
  • Català
  • Čeština
  • Deutsch
  • Español
  • Français
  • Gàidhlig
  • Latviešu
  • Magyar
  • Nederlands
  • Polski
  • Português
  • Português do Brasil
  • Suomi
  • Svenska
  • Türkçe
  • Қазақ
  • বাংলা
  • हिंदी
  • Ελληνικά
  • Yкраї́нська
  • Log in
    Log in
    Have you forgotten your password?
  1. Home
  2. Browse by Author

Browsing by Author "Batlle, Eduard"

Now showing 1 - 1 of 1
Results Per Page
Sort Options
  • No Thumbnail Available
    Item
    The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA
    (2021) Vera-Otarola, Jorge; Castillo-Vargas, Estefania; Angulo, Jenniffer; Barriga, Francisco M.; Batlle, Eduard; Lopez-Lastra, Marcelo
    The capped Small segment mRNA (SmRNA) of the Andes orthohantavirus (ANDV) lacks a poly(A) tail. In this study, we characterize the mechanism driving ANDV-SmRNA translation. Results show that the ANDV-nucleocapsid protein (ANDV-N) promotes in vitro translation from capped mRNAs without replacing eukaryotic initiation factor (eIF) 4G. Using an RNA affinity chromatography approach followed by mass spectrometry, we identify the human RNA chaperone Mex3A (hMex3A) as a SmRNA-3'UTR binding protein. Results show that hMex3A enhances SmRNA translation in a 3'UTR dependent manner, either alone or when co-expressed with the ANDV-N. The ANDV-N and hMex3A proteins do not interact in cells, but both proteins interact with eIF4G. The hMex3A-eIF4G interaction showed to be independent of ANDV-infection or ANDV-N expression. Together, our observations suggest that translation of the ANDV SmRNA is enhanced by a 5'-3' end interaction, mediated by both viral and cellular proteins.

Bibliotecas - Pontificia Universidad Católica de Chile- Dirección oficinas centrales: Av. Vicuña Mackenna 4860. Santiago de Chile.

  • Cookie settings
  • Privacy policy
  • End User Agreement
  • Send Feedback

We collect and process your personal information for the following purposes: Authentication, Preferences, Acknowledgement and Statistics.
To learn more, please read our
privacy policy.

Customize