Browsing by Author "Barros Rodríguez, Fernando Claudio"
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- ItemA Basic 18- Amino Acid Peptide Contains the Polysulfate-Binding Domain Responsible for Activation of the Boar Proacrosin/ Acrosin Syst(2000) Moreno Mauro, Ricardo D.; Barros Rodríguez, Fernando Claudio
- ItemA Trypsin-Like Enzyme Is Present in the Sperm of the Shrimp Rhynchocin Typus(1997) Rios, M.; Barros Rodríguez, Fernando Claudio
- ItemCharacterization of the Functional Domains of Boar Acrosin Involved in Nonenzymatic Binding to Homologous Zona Pellucida Glycoproteins(1998) Crosby, J.; Barros Rodríguez, Fernando Claudio
- ItemDifferential Effects of Polysulphates Between Mouse and Hamster During in Vitro Fertilization(2001) Moreno Mauro, Ricardo D.; Barros Rodríguez, Fernando Claudio
- ItemEarly Steps of Sperm-Egg Interactions During Mammalian Fertilization(1996) Barros Rodríguez, Fernando Claudio
- ItemEfecto del Medio de Cultivo Sobre el Desarrollo Embrionario Bovino in Vitro(2003) De Los Reyes M.; Barros Rodríguez, Fernando Claudio
- ItemEffect of Human Chorionic Gonadotrophin Supplementation During Different Culture Periods on in Vitro Maturation of Canine Oocytes(2005) Reyes M; Barros Rodríguez, Fernando Claudio
- ItemEffect of recombinant boar beta-acrosin on sperm binding to intact zona pellucida during in vitro fertilization(1999) Crosby, J.; Barros Rodríguez, Fernando ClaudioIn a previous paper we demonstrated that boar β-acrosin recombinant proteins were able to bind non-enzymatically to solubilized pig zona pellucida (ZP) glycoproteins. Here we report the participation of boar β-acrosin in the secondary binding of sperm to intact pig ZP. This was achieved by using two boar recombinant proteins: β-acrosin and a mutant of the catalytic site, β-acrosin Ser/Ala222. Assays of binding between the iodinated recombinant β-acrosin and whole ZP showed that this binding could be saturated, was specific, and was stable over time. Using autoradiography, we determined that recombinant β-acrosin bound on the entire surface of the ZP but initially was distributed heterogeneously. This suggests that the ligands for β-acrosin may not be homogeneously distributed on the ZP. To study the contribution of acrosin in sperm secondary binding to the ZP, we preincubated in vitro-matured oocytes with these recombinant proteins and then performed in vitro fertilization assays. Under the experimental conditions used, binding of β-acrosin recombinant proteins did not block sperm penetration. These results suggest that there may be other proteins that participate in the secondary binding, and that these proteins may recognize ligands that are different from those blocked by β-acrosin recombinant proteins.
- ItemEffect of Test-Yolk Upon Human Sperm Acrosome Reaction(1991) Barros Rodríguez, Fernando Claudio; Moreno Bolton, R.
- ItemEgg Coats of the Rock Shrimp Rhynchocinetes Typus: Ultrastructural Characterization and Their Function During Fertilization(2002) Palomino, J.; Moreno Mauro, Ricardo D.; Barros Rodríguez, Fernando Claudio
- ItemEvaluation of Glucose As a Cryoprotectant for Boar Semen(2002) De Los Reyes M.; Barros Rodríguez, Fernando Claudio
- ItemFertilizing Characteristics of Bovine Sperm With Flatened or Indented Acrosomes(2001) Thundathil J; Barros Rodríguez, Fernando Claudio
- ItemFunctional Interactions Between Sulphated Polisaccharides and Proacrosin: Implications in Sperm Binding and Digestion of Zona Pellucida(1999) Moreno Mauro, Ricardo D.; Barros Rodríguez, Fernando Claudio
- ItemGolden hamster perivitelline spermatozoa do not show proacrosin/acrosin at the inner acrosomal space(1992) Yunes, R.; Meléndez Rojel, Jaime Gumercindo; Barros Rodríguez, Fernando Claudio
- ItemHigh rates of survival and fertilization of mouse and hamster oocytes after vitrification in dimethylsulphoxide(1993) Wood, MJ.; Barros Rodríguez, Fernando Claudio; Meléndez Rojel, Jaime Gumercindo
- ItemHuman spermatozoa selected by Percoll gradient or swim-up are equally capable of binding to the human zona pellucida and undergoing the acrosome reaction(1991) Barros Rodríguez, Fernando Claudio; Morales Retamal, Patricio José; Vigil Portales, María del Pilar; Barros Rodríguez, Fernando Claudio; Morales Retamal, Patricio José; Vigil Portales, María del Pilar
- ItemImmunodetection of acrosin during the acrosome reaction of hamster, guinea-pig and human spermatozoa(1992) Barros Rodríguez, Fernando Claudio; Becker C., María Inés; De Ioannes I., Alfredo E.
- ItemImmunolocalization of proacrosin/acrosin in rabbit sperm during acrosome reaction and in spermatozoa recovered from the perivitelline space(1994) Valdivia, M.; Meléndez Rojel, Jaime Gumercindo; Barros Rodríguez, Fernando Claudio
- ItemInhibition of Mouse in Vitro Fertilization by An Antibody Against a Unique 18-Amino Acid Domain in the Polysulfate-Binding Fomain of Proacrosin/Acrosin(2002) Moreno Mauro, Ricardo D.; Barros Rodríguez, Fernando Claudio
- ItemInmunolocalization of Proacrosin/ Acrosin in Bovine Sperm and Sperm Penetration Throught the Zona Pellucida(2000) De Los Reyes M.; Barros Rodríguez, Fernando Claudio