Browsing by Author "Aranda, Eduardo"

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    Antiplatelet, anticoagulant, and fibrinolytic activity in vitro of extracts from selected fruits and vegetables
    (LIPPINCOTT WILLIAMS & WILKINS, 2011) Torres Urrutia, Constanza; Guzman, Luis; Schmeda Hirschmann, Guillermo; Moore Carrasco, Rodrigo; Alarcon, Marcelo; Astudillo, Luis; Gutierrez, Margarita; Carrasco, Gilda; Yuri, Jose A.; Aranda, Eduardo; Palomo, Ivan
    A diet rich in fruits and vegetables is known to decrease the risk of cardiovascular disease. However, the information regarding the antithrombotic activity (antiplatelet, anticoagulant, and fibrinolytic) of fruits and vegetables is scarce. The aim of this study was to assess the antithrombotic activity of extracts from fruits and vegetables widely consumed in central Chile. The study included samples of 19 fruits and 26 vegetables, representative of the local diet. The extracts prepared from each sample included an aqueous (juice or pressed solubles) and/or methanol-soluble fraction. The extracts were evaluated for antiplatelet, anticoagulant, and fibrinolytic activity in vitro at a final concentration of 1 mg/ml. The antiplatelet activity was assessed by platelet aggregation inhibition; anticoagulant activity was measured by the prothrombin time (PT), diluted prothrombin time (dPT), activated partial thromboplastin time (APTT), kaolin clotting time (KCT), and thrombin time. The fibrinolytic effect was determined with the euglobin clot lysis time and fibrin plate methods. Extracts of green beans and tomatoes inhibited platelet aggregation induced by ADP and arachidonic acid, in a concentration-dependent manner. The methanolic extracts of grapes prolonged the PT and dPT. Finally, extracts of raspberry prolonged the APTT and also presented fibrinolytic activity. In conclusion, from a screening that included a variety of fruits and vegetables, we found antiplatelet activity in green beans and tomatoes, anticoagulant activities in grapes and raspberries, whereas fibrinolytic activity was observed only in raspberries. Further investigations are necessary to advance in knowledge of the active compounds of these fruits and vegetables and their mechanisms of action. Blood Coagul Fibrinolysis 22:197-205 (c) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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    High prevalence of bleeders of unknown cause among patients with inherited mucocutaneous bleeding. A prospective study of 280 patients and 299 controls
    (FERRATA STORTI FOUNDATION, 2007) Quiroga, Teresa; Goycoolea, Manuela; Panes, Olga; Aranda, Eduardo; Martinez, Carlos; Belmont, Sabine; Munoz, Blanca; Zuniga, Pamela; Pereira, Jaime; Mezzano, Diego
    Background and Objectives
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    Impact of an Andean breakfast on biochemistry and immunochemistry laboratory tests: an evaluation on behalf COLABIOCLI WG-PRE-LATAM
    (2019) Bajana, Wilson; Aranda, Eduardo; Elena Arredondo, Maria; Michele Brennan-Bourdon, Lorena; Danielle Campelo, Marise; Espinoza, Edgar; Flores, Silvia; Ochoa, Patricia; Vega, Veronica; Varela, Beatriz; Lima-Oliveira, Gabriel
    Introduction: In Andean countries, specifically in Ecuador, a food transition in the population has been observed because of economic growth. The Working Group for Preanalytical Phase in Latin America (WG-PRE-LATAM) of the Latin America Confederation of Clinical Biochemistry (COLABIOCLI) was established in 2017, and its main purpose is to study preanalytical variability and establish guidelines for preanalytical procedures in order to be implemented by clinical laboratories and healthcare professionals in Latin America. The aim of this study on behalf of COLABIOCLI WG-PRE-LATAM was to evaluate whether an Andean breakfast can interfere with routine biochemistry and immunochemistry laboratory tests.
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    Procarboxypeptidase U (TAFI) and the Thr325Ile proCPU polymorphism in patients with hereditary mucocutaneous hemorrhages
    (ELSEVIER, 2009) Matus, Valeria; Willemse, Johan; Quiroga, Teresa; Goycoolea, Manuela; Aranda, Eduardo; Panes, Olga; Pereira, Jaime; Hendriks, Dirk; Mezzano, Diego
    Background: Patients with hereditary mucocutaneous bleeding are difficult to diagnose and many of them fulfill the category of bleeders of unknown cause (BUC). The pathogenic role of hyperfibrinolysis has received little attention, despite the successful use of antifibrinolytic drugs in treating many of these patients. Theoretically, decreased plasma procarboxypeptidase U (proCPU) levels or lower carboxypeptidase U (CPU) stability would result in higher fibrinolytic activity and bleeding tendency.
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    Serotonin secretion by blood platelets: accuracy of high-performance liquid chromatography-electrochemical technique compared with the isotopic test and use in a clinical laboratory
    (2023) Aranda, Eduardo; Iha, Seiki; Solari, Sandra; Rodriguez, David; Romero, Viviana; Villarroel, Luis; Pereira, Jaime; Panes, Olga; Mezzano, Diego
    Background: Mild secretion defects are the most frequent and challenging blood platelet disorders to diagnose. Most d-granule secretion tests lack validation, are not quantitative, or have unreliable response to weak platelet agonists.