Browsing by Author "Andres, M. E."

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    Functional Interplay of Type-2 Corticotrophin Releasing Factor and Dopamine Receptors in the Basolateral Amygdala-Medial Prefrontal Cortex Circuitry
    (2021) Yarur, H. E.; Zegers, J.; Vega-Quiroga, I; Novoa, J.; Ciruela, F.; Andres, M. E.; Gysling, K.
    Background: Basolateral amygdala (BLA) excitatory projections to medial prefrontal cortex (PFC) play a key role controlling stress behavior, pain, and fear. Indeed, stressful events block synaptic plasticity at the BLA-PFC circuit. The stress responses involve the action of corticotrophin releasing factor (CRF) through type 1 and type 2 CRF receptors (CRF1 and CRF2). Interestingly, it has been described that dopamine receptor 1 (D1R) and CRF peptide have a modulatory role of BLA-PFC transmission. However, the participation of CRF1 and CRF2 receptors in BLA-PFC synaptic transmission still is unclear.
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    Short communication: Effect of vitamins E and C on cortisol production by bovine adrenocortical cells in vitro
    (2011) Montalvo, C. P.; Diaz, N. H.; Gaidames, L. A.; Andres, M. E.; Larrain, R. E.
    The aim was to determine if vitamins E and C inhibit the release of cortisol from bovine adrenocortical cells when stimulated with ACTH in vitro. A factorial arrangement of treatments was used to culture bovine adrenocortical cells with different concentrations of vitamins E and C [(+)-alpha-tocopherol at 0, 2.3, and 16 mu M and L-ascorbic acid at 0; 15, and 50 mu M]. After 3 and 7 d of vitamin treatments; cell cultures were stimulated with ACTH (1 nM) for 24 h and the culture medium extracted to measure cortisol released by the cells using HPLC with UV detection. Vitamin E, vitamin C, or their combination did not affect the amount of cortisol released by the adrenal cultures to the media. Cortisol released by the adrenal cultures ranged from 33.6 +/- 6.85 to 49.7 +/- 8.01 nmol per 10(7) cells. The modulation effect of vitamins E and C on the stress response does not take place at the cortex of the adrenal gland.