Browsing by Author "Alvarenga, Augusto Duarte"

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    Fast Fabrication of Multifunctional PCL/Curcumin Nanofibrous Membranes for Wound Dressings
    (2023) Teodoro, Kelcilene Bruna Ricardo; Alvarenga, Augusto Duarte; Rocha Oliveira, Luis Fernando; Marques Chagas, Paulo Augusto; Lopes, Raphael Guimaraes; Andre, Rafaela da Silveira; Mercante, Luiza Amim; Alves, Fernanda; Stringasci, Mirian Denise; Buzza, Hilde Harb; Inada, Natalia Mayumi; Correa, Daniel Souza
    Therapeutic intervention to skin wounds requires coveringthe affectedarea with wound dressings. Interdisciplinary efforts have focusedon the development of smart bandages that can perform multiple functions.In this direction, here, we designed a low cost (U$0.012 per cm(2)) multifunctional therapeutic wound dressing fabricated byloading curcumin (CC) into poly(epsilon-caprolactone) (PCL) nanofibersusing solution blow spinning (SBS). The freestanding PCL/CC bandageswere characterized by distinct physicochemical approaches and weresuccessful in performing varied functions, including controlled releaseof CC, colorimetric indication of the wound conditions, barrier againstmicroorganisms, being biocompatible, and providing a photosensitiveplatform for antimicrobial photodynamic therapy (aPDT). The chemicalnature of PCL and CC and the interactions between these componentsallowed CC to be released for 192 h (ca. 8 days), which could be correlatedwith the Korsmeyer-Peppas model, with a burst release suitableto treat the inflammatory phase. Due to the CC keto-enol tautomerism,an optical indication of the healing status could be obtained usingPCL/CC, which occurred immediately, ranging between red/orange andyellow shades. The effect against pathogenic microorganisms evaluatedby agar disc-diffusion, affected skin wound simulation (ex vivo),and microbial penetration tests demonstrated the ability to blockand inhibit microbial permeation in different environments. The biocompatibilitiesof PCL and PCL/CC were verified by in vitro cytotoxicity study, whichdemonstrated that cell viabilities average above 94 and 96% for humandermal fibroblasts. In addition, the proposed bandage responded toaPDT applied to an in vivo assay, showing that, when irritated, PCL/CCwas able to reduce the bacteria present on the real wound of mice.In summary, our findings demonstrate that using PCL and CC to producenonwovens by the SBS technique offers potential for the rapid fabricationof biocompatible and multifunctional wound dressings, paving the wayfor large-scale production and utilization of such dressings in thetreatment of skin wounds.