Browsing by Author "Agosin Trumper, Eduardo"
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- ItemDevelopment of Reproducible high cell density operations for Heterologous Saccharomyces cerevisiae fermentation processes(2024) Ibañez Espinel, Francisco; Pérez C., José Ricardo; Agosin Trumper, Eduardo; Pontificia Universidad Católica de Chile. Escuela de IngenieríaSaccharomyces cerevisiae (SC) is widely used in biotechnology to produce high-value indus trial compounds. Among its advantages are: a known genomic sequence, defined nutritional re quirements, and the ability to grow at high cellular densities. However, reaching densities above 100 g/L is challenging due to the Crabtree effect or metabolic overflow, which can result in ethanolproduction, an inhibitor of cell growth.To address this issue, automatic control strategies have been developed aiming to keep growthbelow the critical specific growth rate. These strategies largely rely on stationary or dynamicmodels, either based on first principles or black-box approaches. In general, the models strive toaccurately reflect the actual behavior of biomass and key metabolites associated with SC growth,emphasizing precision and simplicity.Despite the existence of experimentally validated advanced control schemes, consistently reach ing biomass concentrations of 100 g/L has not been achieved. Furthermore, many of these strate gies employ instrumentation not typically available in industrial bioreactors, either due to their highcost or insufficient reliability. Therefore, to optimize SC cultivation, it’s essential to develop ad vanced automatic control strategies using reliable measurements commonly available in industrialbioreactors (O2 gas, CO2 gas, pH, T, DO, among others).The primary objective of this thesis was to design an automatic operating system for manag ing SC cultures, aiming to achieve high cellular densities consistently and reproducibly. Initially,the development of a dynamic model was proposed to simulate the various observable metabolicstates in an SC culture with cellular densities above 100 g/L. This model was later integrated withadvanced control techniques, considering real conditions and potential measurement noise. Fi nally, a state observer was designed to estimate metabolites not directly measurable, crucial foroptimizing the controller. The production of carotenogenic recombinant SC strains was used as acase study, providing a relevant platform for effective and reproducible operation demonstration ofhigh-density cultures.
- ItemReliable calibration and validation of phenomenological and hybrid models of high-cell-density fed-batch cultures subject to metabolic overflow(2024) Ibáñez Espinel, Francisco; Puentes Cantor, Hernán Felipe; Barzaga Martell, Lisbel; Saa Higuera, Pedro; Agosin Trumper, Eduardo; Perez Correa, José RicardoFed-batch cultures are the preferred operation mode for industrial bioprocesses requiring high cellular densities. Avoids accumulation of major fermentation by-products due to metabolic overflow, increasing process productivity. Reproducible operation at high cell densities is challenging (> 100 gDCW/L), which has precluded rigorous model evaluation. Here, we evaluated three phenomenological models and proposed a novel hybrid model including a neural network. For this task, we generated highly reproducible fedbatch datasets of a recombinant yeast growing under oxidative, oxygen-limited, and respiro-fermentative metabolic regimes. The models were reliably calibrated using a systematic workflow based on pre-and post-regression diagnostics. Compared to the best-performing phenomenological model, the hybrid model substantially improved performance by 3.6- and 1.7-fold in the training and test data, respectively. This study illustrates how hybrid modeling approaches can advance our description of complex bioprocesses that could support more efficient operation strategies
- ItemSistema y método para recuperar aromas en una instalación con una pluralidad de cubas fermentativas para preparar bebidas, donde dicho sistema comprende: al menos un sistema de conexión para transportar fluidos, conectado a la salida de gases de dichas cubas fermentativas y al menos un equipo recuperador de aroma conectado a dicho sistema de conexión y conectado a al menos un recipiente para almacenar aromas, y válvulas automáticas conectadas a dicha pluralidad de cubas fermentativas y un medio de control (España)Agosin Trumper, Eduardo; Pérez, Ricardo; Delgado, Mariana; Capitain, Chloe; Lezaeta, Álvaro; Alemparte Benavente, Eduardo Andrés; Benavente Pereira, José Miguel; Heiremans Bunster, Jorge Alberto
- ItemSistema y método para recuperar aromas en una instalación con una pluralidad de cubas fermentativas para preparar bebidas, donde dicho sistema comprende: al menos un sistema de conexión para transportar fluidos, conectado a la salida de gases de dichas cubas fermentativas y al menos un equipo recuperador de aroma conectado a dicho sistema de conexión y conectado a al menos un recipiente para almacenar aromas, y válvulas automáticas conectadas a dicha pluralidad de cubas fermentativas y un medio de control (Unitaria Europea, concesión n° 3564352)Agosin Trumper, Eduardo; Pérez, Ricardo; Delgado, Mariana; Capitain, Chloe; Lezaeta, Álvaro; Alemparte Benavente, Eduardo Andrés; Benavente Pereira, José Miguel; Heiremans Bunster, Jorge Alberto