Browsing by Author "Acuña-Castillo, C"

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    Aleph2, a Suspected Anxiolytic and Putative Hallucinogenic Phenylisopropylamine Derivative, Is a 5-Ht2a and 5-Ht2c Receptor Agonist
    (2000) Acuña-Castillo, C; Scorza, C; Reyes-Parada, M; Cassels, BK; Huidobro-Toro, JP
    To assess the pharmacodynamic profile of ALEPH-2, a phenylisopropylamine derivative with alleged anxiolytic and hallucinogenic properties,Xenopus laevis oocytes were microinjected with either of the rat cRNA for the 5-HT2A or the 5-HT2C receptor. Concentration-response curves were obtained following the exposure of the oocytes to varying concentrations of either ALEPH-2 or 5-hydroxy-tryptamine (5-HT) for 10 s. ALEPH-2 is a partial agonist on the 5-HT,,receptor with a similar potency to 5-HT. In contrast, ALEPH-2 is a full 5-HT2C receptor agonist and is about 15-fold less potent than 5-HT. Pre-application of 1 muM ritanserin antagonized the responses induced by 5-HT and ALEPH-2 to the same extent; however, the 5-HT2A receptor is more sensitive to ritanserin blockade than the 5-HT2C receptor. (C) 2000 Elsevier Science Inc. All rights reserved.
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    Differences in potency and efficacy of a series of phenylisopropylamine/phenylethylamine pairs at 5-HT2A and 5-HT2C receptors
    (2002) Acuña-Castillo, C; Villalobos, C; Moya, PR; Sáez, P; Cassels, BK; Huidobro-Toro, J
    1 The pharmacological profile of a series of (+/-)-2,5-dimethoxy-4-(X)-phenylisopropylamines (X = I, Br, NO2, CH3, or H) and corresponding phenylethylamines, was determined in Xenopus laevis oocytes injected with cRNA coding for rat 5-HT2A or 5-HT2C receptors. The efficacy and relative potency of these drugs were determined and compared to classical 5-HT2 receptor agonists and antagonists.
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    Heavy metals modulate the activity of the purinergic P2X4 receptor
    (2005) Coddou, C; Lorca, RA; Acuña-Castillo, C; Grauso, M; Rassendren, F; Huidobro-Toro, JP
    To further characterize the nature of the regulatory metal-binding sites of the rat P2X(4) receptor, several transition heavy metals were tested to examine their ability to mimic the facilitator action of zinc or the inhibitory action of copper. cDNA coding for the rat P2X(4) receptor was injected into Xenopus laevis oocytes; the two-electrode voltage-clamp technique was used to measure and quantify the ATP-evoked currents in the absence or presence of the metals. Cadmium facilitated the ATP-gated currents in a reversible and voltage-independent manner; maximal potentiation occurred within less than 1 min.
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    Zinc and copper modulate differentially the P2X4 receptor
    (2000) Acuña-Castillo, C; Morales, B; Huidobro-Toro, JP
    The rat ATP P2X(4) receptor was expressed in Xenopus laevis oocytes to assess the effect of zinc and copper as possible regulators of purinergic mechanisms. ATP applied for 20 s evoked an inward cationic current with a median effective concentration (EC50) of 21.4 +/- 2.8 mu M and a Hill coefficient (n(H)) of 1.5 +/- 0.1. Coapplication of ATP plus 10 mu M zinc displaced leftward, in a parallel fashion, the ATP concentration-response curve, reducing the EC50 to 8.4 +/- 1.8 mu M (p < 0.01) without altering the receptor n(H). The zinc potentiation was fast in onset, easily reversible, and voltage-independent and did not require metal preexposure. The zinc EC50 was 2-5 mu M, with a bell-shaped curve. At concentrations of 100-300 mu M, zinc produced less potentiation, and at 1 mM, it inhibited 50% the ATP current. The effect of zinc was mimicked by cadmium, in contrast, copper inhibited the ATP-evoked currents in a time- and concentration-dependent fashion, reducing the maximal current (I-max) without altering the EC50. The copper-induced inhibition was slow in onset, slowly reversible, and voltage-independent. Whereas coapplication of 300 mu M copper plus ATP reduced I-max to 36.2 +/- 5%, the coapplication of, or 60-s preexposure by, 10 mu M copper reduced I-max to 79 +/- 9.2% (p < 0.05) and 39.6 +/- 8.7% (p < 0.01), respectively. The inhibition was noncompetitive in nature and mimicked by mercury. Cobalt, barium, and manganese did not modify significantly the ATP-evoked current, demonstrating metal specificity. The simultaneous 1-min preapplication of both metals revealed that the 10 mu M zinc-induced potentiation was obliterated by 10 mu M copper, whereas 30 mu M copper not only reduced the potentiation, but inhibited the ATP response. Following coapplication of both metals for 20 s with ATP, at least 100 mu M copper was required to counteract the 10 mu M zinc-induced potentiation. The simultaneous preincubation with both metals provided evidence for a noncompetitive interaction. We hypothesize the existence of metal binding site(s), which are most likely localized in the extracellular domain of the P2X(4) receptor structure. These sites are selective and accessible to extracellular metal applications and bind micromolar concentrations of metals. The present results are compatible with the working hypothesis that trace metals, such as copper and zinc, are physiological modulators of the P2X(4) receptor. The modulation of brain purinergic transmission by physiologically and toxicologically relevant trace metal cations is highlighted.