Browsing by Author "Acevedo, Keryma"

Now showing 1 - 4 of 4
  • Thumbnail Image
    Item
    Initial combination versus early sequential standard therapies for Infantile Epileptic Spasms Syndrome-Feedback from stakeholders
    (2024) Ramani, Praveen Kumar; Briscoe Abath, Christina; Donatelli, Stephanie; Hadjinicolaou, Aristides; Vega Toro, Sebastian; Acevedo, Keryma; Astorga, Karina Rosso; Parbhoo, Kaajal; Singh, Avantika; Catenaccio, Eva; Jain, Puneet; Sahu, Jitendra Kumar; Samanta, Debopam; Harini, Chellamani
  • No Thumbnail Available
    Item
    Micronutrients intake in patients with refractory epilepsy with ketogenic diet treatment
    (2024) Velandia, Silvia; Astudillo, Patricio; Acevedo, Keryma; Le Roy, Catalina
    The Ketogenic Diet (KD) is a non-pharmacological strategy for drug-resistant epilepsy (DRE) and inborn errors of metabolism (Glut-1 deficiency) management. KD is characterized by being restrictive, affecting micronutrient intake. There are different modalities of KD in which food intake and nutritional deficiencies vary. Objective: To determine the micronutrient intake in different KD modalities. Patients and Method: Observational, cross-sectional study with patients diagnosed with DRE and Glut-1 deficiency. The dietary intake of 21 micronutrients was evaluated, and analyzed according to KD modality [Classic, Modified Atkins Diet (MAD)], use of special formula, and adequacy of recommended dietary intake (RDI) according to age and sex, defining < 75% as deficient. Results: 19 patients were evaluated, median age 62 months (IQR: 20.5-79), 12/19 (63.2%) male, 13/19 (68.4%) eutrophic, 5/19 (26.3%) gastrostomy users, 10 (52.6%) MAD modality, use of special formula 7/19 (36.8%). Micronutrient deficiencies were found in 16/21 (76.2%) in the classic diet and 9/16 (42.9%) in the MAD. The intake of vitamin D, B2, B12, sodium, phosphorus, zinc, and selenium was significantly lower in the classic diet than in DMA, the median intake adequacy of vitamins A, C, D, E, and K was > 100%. The use of special formula manages to complete the requirements in MAD. Conclusions: The micronutrient intake in the different KD modalities is low for most of them, being the MAD with formula the one that presented a more adequate micronutrient intake. These results should be considered in nutritional follow-up and supplementation planning.
  • No Thumbnail Available
    Item
    Micronutrients intake in patients with refractory epilepsy with ketogenic diet treatment
    (2024) Velandia, Silvia; Astudillo, Patricio; Acevedo, Keryma; Le Roy, Catalina
    The Ketogenic Diet (KD) is a non-pharmacological strategy for drug-resistant epilepsy (DRE) and inborn errors of metabolism (Glut-1 deficiency) management. KD is characterized by being restrictive, affecting micronutrient intake. There are different modalities of KD in which food intake and nutritional deficiencies vary. Objective: To determine the micronutrient intake in different KD modalities. Patients and Method: Observational, cross-sectional study with patients diagnosed with DRE and Glut-1 deficiency. The dietary intake of 21 micronutrients was evaluated, and analyzed according to KD modality [Classic, Modified Atkins Diet (MAD)], use of special formula, and adequacy of recommended dietary intake (RDI) according to age and sex, defining < 75% as deficient. Results: 19 patients were evaluated, median age 62 months (IQR: 20.5-79), 12/19 (63.2%) male, 13/19 (68.4%) eutrophic, 5/19 (26.3%) gastrostomy users, 10 (52.6%) MAD modality, use of special formula 7/19 (36.8%). Micronutrient deficiencies were found in 16/21 (76.2%) in the classic diet and 9/16 (42.9%) in the MAD. The intake of vitamin D, B2, B12, sodium, phosphorus, zinc, and selenium was significantly lower in the classic diet than in DMA, the median intake adequacy of vitamins A, C, D, E, and K was > 100%. The use of special formula manages to complete the requirements in MAD. Conclusions: The micronutrient intake in the different KD modalities is low for most of them, being the MAD with formula the one that presented a more adequate micronutrient intake. These results should be considered in nutritional follow-up and supplementation planning.
  • No Thumbnail Available
    Item
    Prophylactic treatment with the c-Abl inhibitor, neurotinib, diminishes neuronal damage and the convulsive state in pilocarpine-induced mice
    (Elsevier B.V., 2024) Chandía Cristi, América Valeska; Gutiérrez García, Daniela A.; Dulcey, Andrés E.; Lara, Marcelo; Vargas Rojas, Lina Marcela; Lin, Yi-Han; Jiménez Muñoz, Pablo Salvador; Larenas Barrera, Gabriela Paz; Xu, Xin; Wang, Amy; Owens, Ashley; Dextras, Christopher; Chen, YuChi; Pinto, Claudio; Marín Marín, Tamara Alejandra; Almarza Salazar, Hugo Alcester; Acevedo, Keryma; Cancino Lobos, Gonzalo Ignacio; Hu, Xin; Rojas, Patricio; Ferrer, Marc; Southall, Noel; Henderson, Mark J.; Zanlungo Matsuhiro, Silvana; Marugan, Juan J.; Álvarez Rojas, Alejandra
    The molecular mechanisms underlying seizure generation remain elusive, yet they are crucial for developing effective treatments for epilepsy. The current study shows that inhibiting c-Abl tyrosine kinase prevents apoptosis, reduces dendritic spine loss, and maintains N-methyl-D-aspartate (NMDA) receptor subunit 2B (NR2B) phosphorylated in in vitro models of excitotoxicity. Pilocarpine-induced status epilepticus (SE) in mice promotes c-Abl phosphorylation, and disrupting c-Abl activity leads to fewer seizures, increases latency toward SE, and improved animal survival. Currently, clinically used c-Abl inhibitors are non-selective and have poor brain penetration. The allosteric c-Abl inhibitor, neurotinib, used here has favorable potency, selectivity, pharmacokinetics, and vastly improved brain penetration. Neurotinib-administered mice have fewer seizures and improved survival following pilocarpine-SE induction. Our findings reveal c-Abl kinase activation as a key factor in ictogenesis and highlight the impact of its inhibition in preventing the insurgence of epileptic-like seizures in rodents and humans.