Browsing by Author "ABEIJON, C"

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    DIFFERENTIAL ASSOCIATION AND DISTRIBUTION OF ACETYLCHOLINESTERASE AND BUTYRYLCHOLINESTERASE WITHIN RAT-LIVER SUBCELLULAR ORGANELLES
    (1990) PERELMAN, A; ABEIJON, C; HIRSCHBERG, CB; INESTROSA, NC; BRANDAN, E
    Rat liver cholinesterases were found to share properties and characteristics with those expressed in cholinergic tissues. The distribution and presence of different molecular forms of cholinesterases in different subcellular organelles of rat liver were studied. The rough and smooth endoplasmic reticulum and Golgi apparatus were enriched in the G4 molecular form of acetylcholinesterase (AChE) (relative to the G2 molecular form), while the inverse was found in the plasma membrane. The interaction of these molecular forms of AChE with the Golgi membrane was studied in detail. Approximately one-half of the G4 form was free within the lumen while the remainder was an intrinsic membrane protein; all the G2 molecular form was anchored to the membrane via phosphatidylinositol. Only the G1 and G2 molecular forms of butyrylcholinesterase (BuChE) were found in the above subcellular organelles; both molecular forms were soluble within the lumen of Golgi vesicles. These results indicate that rat liver expresses several molecular forms of AChE which have multiple interactions with membranes and that liver is unlikely to be the source of the G4 form of BuChE present in high concentration in the plasma.