Improved stability and activity of laccase through <i>de novo</i> and post-synthesis immobilization on a hierarchically porous metal-organic framework (ZIF-8)

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dc.contributor.authorXu, Ran
dc.contributor.authorZhang, Xujie
dc.contributor.authorZelekew, Osman Ahmend
dc.contributor.authorSchott, Eduardo
dc.contributor.authorWu, Yi-nan
dc.date.accessioned2025-01-20T20:11:02Z
dc.date.available2025-01-20T20:11:02Z
dc.date.issued2023
dc.description.abstractPorous materials such as metal-organic frameworks (MOFs) are considered to be suitable materials for immobilizing enzymes to improve their stability. However, conventional MOFs reduce the enzymes' catalytic activity due to difficulties with mass transfer and diffusing reactants after their micropores are occupied by enzyme molecules. To address these issues, a novel hierarchically structured zeolitic imidazolate framework-8 (HZIF-8) was prepared to study the effects of different laccase immobilization approaches such as the post-synthesis (LAC@HZIF-8-P) and de novo (LAC@HZIF-8-D) immobilization of catalytic activities for removing 2,4-dichlorophenol (2,4-DCP). The results showed higher catalytic activity for the laccase-immobilized LAC@HZIF-8 prepared using different methods than for the LAC@MZIF-8 sample, with 80% of 2,4-DCP removed under optimal conditions. These results could be attributable to the multistage structure of HZIF-8. The LAC@HZIF-8-D sample was stable and superior to LAC@HZIF-8-P, maintaining a 2,4-DCP removal efficiency of 80% after three recycles and demonstrating superior laccase thermostability and storage stability. Moreover, after loading with copper nanoparticles, the LAC@HZIF-8-D approach exhibited a 2,4-DCP removal efficiency of 95%, a promising finding for its potential use in environmental purification.
dc.fuente.origenWOS
dc.identifier.doi10.1039/d3ra01571h
dc.identifier.eissn2046-2069
dc.identifier.urihttps://doi.org/10.1039/d3ra01571h
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92115
dc.identifier.wosidWOS:001002294900001
dc.issue.numero25
dc.language.isoen
dc.pagina.final17201
dc.pagina.inicio17194
dc.revistaRsc advances
dc.rightsacceso restringido
dc.titleImproved stability and activity of laccase through <i>de novo</i> and post-synthesis immobilization on a hierarchically porous metal-organic framework (ZIF-8)
dc.typeartículo
dc.volumen13
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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