PRAME/MELAN-A double immunostaining as a diagnostic tool for conjunctival melanocytic lesions: A South American experience

Simple item page
dc.contributor.authorCarvajal, Pedro
dc.contributor.authorZoroquiain, Pablo
dc.date.accessioned2025-01-20T17:28:44Z
dc.date.available2025-01-20T17:28:44Z
dc.date.issued2023
dc.description.abstractIntroduction: PRAME (PReferentially expressed Antigen in Melanoma) is an antigen that is predominantly expressed in human melanomas. In cutaneous melanocytic lesions, PRAME expression is associated with malignancy. The objective of this study was to evaluate the co-expression of PRAME and Melan A to evaluate their diagnostic value in different conjunctival melanocytic lesions (CML).Methods: 37 CML (23 nevi, 9 primary acquired melanosis (PAM), and 5 conjunctival melanomas) were evaluated by immunohistochemistry for PRAME and Melan-A. The percentage of melanocytic cells co-expressing PRAME and Melan-A was qualitatively evaluated as follows: negative, 0%; 1 +, 1-25%; 2 +, 26-50%; 3 +, 51-75% and 4 + , >= 76%. Results: Of the invasive melanoma cases, 80% showed a 4 + pattern of marking, whereas 20% showed a 3 + pattern. 11% of the PAMs showed a 4 + pattern and 88.9% showed a 1 + pattern. All the nevi showed a 1 + pattern. The sensitivity and specificity of PRAME 4 + for differentiating high-grade CML from the benign and low-grade grouped CML are 93% and 100%, respectively.Conclusion: PRAME/MELAN-A double immunostain is particularly useful to differentiate benign from malignant conjunctival melanocytic lesions.
dc.description.funder2021 fondos concursables becarios-residentes UC from Pontificia Universidad Catolica de Chile
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.prp.2023.154776
dc.identifier.eissn1618-0631
dc.identifier.issn0344-0338
dc.identifier.urihttps://doi.org/10.1016/j.prp.2023.154776
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91591
dc.identifier.wosidWOS:001083617600001
dc.language.isoen
dc.revistaPathology research and practice
dc.rightsacceso restringido
dc.subjectImmunohistochemistry
dc.subjectHMB45
dc.subjectPRAME
dc.subjectMelanocytic
dc.subjectConjunctival
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePRAME/MELAN-A double immunostaining as a diagnostic tool for conjunctival melanocytic lesions: A South American experience
dc.typeartículo
dc.volumen250
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas