L-PRF Secretome from Both Smokers/Nonsmokers Stimulates Angiogenesis and Osteoblast Differentiation In Vitro

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dc.contributor.authorRios, Susana
dc.contributor.authorGonzalez, Lina Gabriela
dc.contributor.authorSaez, Claudia Gilda
dc.contributor.authorSmith, Patricio Cristian
dc.contributor.authorEscobar, Lina
dc.contributor.authorMartinez, Constanza Eugenia
dc.date.accessioned2025-01-20T16:19:43Z
dc.date.available2025-01-20T16:19:43Z
dc.date.issued2024
dc.description.abstractLeukocyte and Platelet-Rich Fibrin (L-PRF) is part of the second generation of platelet-concentrates. L-PRF derived from nonsmokers has been used in surgical procedures, with its beneficial effects in wound healing being proven to stimulate biological activities such as cell proliferation, angiogenesis, and differentiation. Cigarette smoking exerts detrimental effects on tissue healing and is associated with post-surgical complications; however, evidence about the biological effects of L-PRF derived from smokers is limited. This study evaluated the impact of L-PRF secretome (LPRFS) derived from smokers and nonsmokers on angiogenesis and osteoblast differentiation. LPRFS was obtained by submerging L-PRF membranes derived from smokers or nonsmokers in culture media and was used to treat endothelial cells (HUVEC) or SaOs-2 cells. Angiogenesis was evaluated by tubule formation assay, while osteoblast differentiation was observed by alkaline phosphatase and osterix protein levels, as well as in vitro mineralization. LPRFS treatments increased angiogenesis, alkaline phosphatase, and osterix levels. Treatment with 50% of LPRFS derived from smokers and nonsmokers in the presence of osteogenic factors stimulates in vitro mineralization significantly. Nevertheless, differences between LPRFS derived from smokers and nonsmokers were not found. Both LPRFS stimulated angiogenesis and osteoblast differentiation in vitro; however, clinical studies are required to determine the beneficial effect of LPRFS in smokers.
dc.description.funderNational Agency for Research and Development ANID from the Chilean Government: program Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT)
dc.fuente.origenWOS
dc.identifier.doi10.3390/biomedicines12040874
dc.identifier.eissn2227-9059
dc.identifier.urihttps://doi.org/10.3390/biomedicines12040874
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90703
dc.identifier.wosidWOS:001210670800001
dc.issue.numero4
dc.language.isoen
dc.revistaBiomedicines
dc.rightsacceso restringido
dc.subjectleukocyte- and platelet-rich fibrin
dc.subjectsecretome
dc.subjectcigarette smoking
dc.subjectangiogenesis
dc.subjectosteoblast
dc.subjectcell differentiation
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleL-PRF Secretome from Both Smokers/Nonsmokers Stimulates Angiogenesis and Osteoblast Differentiation In Vitro
dc.typeartículo
dc.volumen12
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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